Protocols we use in the facility (updated Dec 2024)
- S-Trap digestion - micro protocol (<50ug protein)
- More digestion protocols from S-Trap (>50ug, Plasma, Urine samples)
- Tissue and Cell lysis protocol
- In Solution Digestion Protocol (Urea In-Solution)
- Digestion off Magnetic Beads (IP)
- Using iRT retention time standards in Skyline
- Manual MagReSyn Hydroxyl PAC digestion
- KingFisher MagReSyn Hydroxyl PAC digestion - also has Phospho and TMT
- In Gel Digest
We also recommend:
Our Videos - Sign into Google Drive
- Follow the link for videos illustrating how protocols are performed in our lab, adapted from the above protocols
Other Nice Protocols
- Ingel Digestion Protocol 2
- Precipitation of Proteins (compares various protein precipitation methods)
Examinations of Protocols by Lab Core Members
- Digestion of Mouse Liver using the Opentrons Flex and S-Trap 96 well plate: OTFlex-load-s-trap.pptx
- Examining reproducibility of loading Evotips by hand and the OT-2 using DDM: DDM-Opentrons UC Davis Proteomics 2024.pptx
- For final findings please see the posters section:
- Initial digestion comparison between DDM, S-Trap, and Kingfisher PAC: Digestion Comparisons.pptx
- Initial digestion evaluation of Kingfisher PAC: KingFisher Apex Beads.pptx
- Examining Plasma/Serum using S-Trap, Depletion, and MagReSyn SAX beads: Plasma protein sample prep UC Davis 2023.pptx