Protocols we use in the facility (updated Dec 2024)
- S-Trap micro protocol (<50ug protein)
- More protocols from S-Trap (>50ug, Plasma, Urine samples)
- Tissue and Cell lysis protocol
- In Solution Digestion Protocol (Urea In-Solution)
- Digestion off Magnetic Beads (IP)
- Using iRT retention time standards in Skyline
- Manual MagReSyn Hydroxyl PAC digestion
- KingFisher MagReSyn Hydroxyl PAC digestion - also has Phospho and TMT
- In Gel Digest
We also recommend:
Other Nice Protocols
- Ingel Digestion Protocol 2
- Precipitation of Proteins (compares various protein precipitation methods)
