Prices

I know pricing should not be difficult

Unfortunately, it's a bit tricky if to charge accurately for the work we perform. 

 

All samples are a bit different and take different amounts of time to work on . Almost all of the variability (in your results & in the pricing) is due to the protein extraction and sample prep. All samples are a bit different and require different amount of time to prepare. LC-MS runs are charged per hour.

We can give you an estimate and you can cut down the costs by doing a lot of the work yourself and using shorter LC-MS gradients.

Doing the protein extraction yourself is not that difficult

 

We use S-traps from Protifi.com as they work with a wide variety of samples and we can semi automate the digestion using our Tecan A200

Essentially Follow these steps and stop After Step 4

s-trap protocol

You should also do a BCA at this point as knowing the protein concentration is very beneficial. But if you can estimate accurately using past knowledge, this works too

 

The entire protocol is here

 

and there are some great troubleshooting tips at the bottom and here 

 

You can do the whole protocol your self and save even more money , plus it's fun

 

The above protocol is for 100-300 ug of starting protein. If you have less you can use this protocol 

but steps 1-4 are essentially the same

This protocol works well too 

There are also specific protocols for things like Urine or Plasma here

If you don't want to use S-traps we recommend these 

Thermo Easy pep kits

or

Preomics Kits

 

 

 

Contact Us


Any Questions? Contact Us.
Phone: (530) 754-9474 (please email us first)
Text: (530)771-7055
Email: proteomics@ucdavis.edu
Twitter DM: @ucdproteomics

 

Our shipping address is:
Proteomics Core Facility
Room 1414 GBSF
451 E. Health Sciences Dr.
University of California
Davis, CA 95616

 

Please do not use US Mail to send samples as they will likely be delayed, potentially affecting the quality of your sample.