FAQ

First, email the Core or personnel you have worked with before.

You will be asked if you have a PPMS account, have properly labeled your samples, and will need to input a submission form.

See: Submissions

It depends on the experiment and status of our LCMS, however:

The turnaround time for peptide samples is usually 2 weeks for on-campus users.

The turnaround time for protein samples is usually 3 weeks for on-campus users.

See Prices for our typical per-sample cost.

Generally, these prices decrease when more samples are sent as a set and will be mentioned when discussing with personnel before submitting a sample set.

There are multiple reasons why:

Western Blot may be more sensitive than the machine.

Western Blot may not be binding the protein that you think it is.

Do all the steps mentioned in Submissions before submitting your sample sheet.

When submitting your sample sheet, answer all possible fields. The more crucial ones include buffer composition, organism, and the UniProt ID.

Do not send samples with US mail (USPS). Instead use Fedex (within the US) or DHL.

Always let us know that you have sent samples. Do not ship on a Friday because receiving will not be open when they arrive.

It depends on the type of sample. Generally, dry peptides can be sent on ice packs or at room temperature. Do not send beads frozen, dry, or on dry ice as it breaks the beads and causes contamination.

There are several required fields when filling out the sample sheet.

Either the submitter or the PI needs to have an active PPMS account associated with the email entered in the sheet. This is both a required field and one that is checked before the sheet may be submitted.