DIA is now our Default for Quantitative proteomic profiling. It's much better than our older Label free methods we have used in teh past and is much cheaper and faster than TMT (see below)
- Can be very inexpensive (< 50 / sample)
- Good proteome Depth!
- Sensitive (down to single cell level is possible)
- Fast Turn around time if you do the sample prep
- Data analysis can be easier than other methods
- Does not work well for some esoteric PTM's
- Sometimes does not work well for very complex multispecies proteomes
For More info see this presentation
Some of our recent Example Publications
Still generates really good data!
- Good quantitative Data
- Good proteome Depth with peptide Fractionation
- Optimally should be analyzed on our most expensive mass spectrometer
- Turn around time is long if you want us to do the labeling
TMT is a reporter ion isotope labeled balanced set of tag’s (currently 18) .
- digest proteins into peptides,
- label with TMT, which labels free amines (K and N-term)
- Mix your samples together (create a multiplex)
- Fractionate your samples using high pH fractionation if your sample is very complex (lysate for example)
- Analyze your peptide fractions using SPS MS3 on our Fusion Lumos
Some of our Example Publications
Here is a presentation of a dataset we did recently. The author of the above publication helps us with the analysis. Make sure you read the notes as most of the detailed info is located there.