Enrichment with Ceres Nanosciences Nanotrap PEAK

Using the Nanotrap Protein Enrichment Kit from Ceres Nanosciences, we can enhance the identification of proteins. Our example data shows how the change in abundance of immunoglobulins and albumin allows us to see more in the same samples over neat digestion. The table shows the increased identification of proteins over neat digestion.

Ranked Abundance from Spectronaut of 5 plasma samples digested NEAT using Protifi S-Trap. Highlighted are the top ranked albumin and Immunoglobulins at 1, 2 and 4.
Ranked Abundance from Spectronaut of 5 plasma samples digested NEAT using Protifi S-Trap. Highlighted are the top ranked albumin and Immunoglobulins at 1, 2 and 4.
Ranked Abundance from Spectronaut of 5 plasma samples enriched using Ceres Nanotrap PEAK. Highlighted are the same albumin and immunoglobulin proteins as in the neat. These are greatly reduced in ranked abundance in comparison to neat digestion.
Ranked Abundance from Spectronaut of 5 plasma samples enriched using Ceres Nanotrap PEAK. Highlighted are the same albumin and Immunoglobulins as neat, but the ranked abundance is much decreased. The first immunoglobulin shows up at rank 95.
Example Enrichment Data of Various Sample Types
Sample TypeAve Proteins from NeatAve Proteins from Nanotrap PEAK
Human Plasma5302281
Horse Synovial Fluid9011860
Cell Media 1293560*
Cell Media 210913193
Cell Media 38941932

*enrichment performed with 1/4 the volume of cell media prescribed by protocol

Figures from data from samples processed with the Ceres Nanotrap PEAK Discovery Kit - pdf