Targeted Proteomics

Prices:
Targeted Proteomics Prices
Service University of California Non-Profit For-Profit
Targeted Analysis (QQQ) $70.00 $108.00 $128.00
Method Development $70.00 $108.00 $128.00
* These are bundled prices, for our official prices please see http://proteomics.ucdavis.edu/prices-jan-2015
You may notice the actual service is considerably less expensive than our Protein ID service. The reason for this is that we did not include any data analysis, or method development in the targeted picservice cost. Some projects take a lot of method development and some do not, so we felt it was fair to charge for that seperatly. Also once you have the method developed, you usually only have to pay for the LC-MS/MS. Please call if you have any questions
Targeted Proteomics targets specific peptides in a complex mixture of other peptides and determines their presence (if they are above our detection limit) and quantity in one sample or across multiple samples. You can think of it as a mass spectrometer version of a western blot, but far more accurate and not requiring any antibodies.This analysis usually utilizes a triple quadrupole mass spectrometer (QQQ) which has been used for a long time to quantitate small molecules. Only recently has it been utilized for peptides. Parallel Reaction monitoring (PRM) and data independent analysis (DIA) are also options and can be far cheaper than developing a method for a QQQ…but preliminary runs are also an important part of that analysis as well.
Here are some examples where you would utilize this type of analysis.
  • If you want to verify that your knockdown target is knocked down.
  • If you want to know the quantity of a peptide/protein  in a complex mixture of other peptides or proteins (PSA in blood serum, oxytocin in cerebral spinal fluid, a protein in a cell lysate or cell fraction)
  • If you want to see if a specific protein is differentially expressed across samples or during a time course experiment.

This method actually “targets” peptides enzymatically digested from your target protein, so it is important to pick your peptides and method conditions carefully. Please come and talk to us if you are considering this type of experiment. This analysis also requires a good bit of preliminary analysis to ensure the method is robust and quantitative and thus cannot usually be done with 1 or 2 mass spectrometry runs.

Here is a nice collection of recent Targeted proteomics applications and research papers
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