We offer a yeast one hybrid screening service between promoters and collections of Arabidopsis or maize transcription factors. Three collections of transcription factors are available:
i. A root stele-expressed collection of 800 Arabidopsis transcription factors (Gaudinier, Zhang et al. 2011, Li, Gaudinier et al. 2014) TFLIST 12-10-2014 CLM-1 (-850 root expressed TFs);
ii. A complete collection of 2000 Arabidopsis transcription factors (Pruneda-Paz, Breton et al. 2014) TFLIST 11-30-2015 (full Arabidopsis TF collection); and
iii. A collection of 500 maize transcription factors (Burdo, Gray et al. 2014) Maize TF Masterlist 3-2016.
Once the number of promoters to be screened and an estimate of cost is provided and agreed upon between the user and the core, an estimate of time to both the start and end of the screen will be provided. The user will recombine their promoter(s) into a Gateway pDONR-P4P1 plasmid or a Gateway 5’TOPO plasmid followed by the pMW2 and pMW3 yeast reporter plasmids. These will then be provided to the UC Davis Core. The UC Davis core completes yeast transformation, auto-activation tests and genotyping in addition to mating-based screens. Positive interactions are recorded for LacZ and HIS3 reporters.
|Service||University of California||Non-Profit/ Education||For-Profit/ Industry|
|Y1H Screening of 1-14 Promoters/Arabidopsis 800||$6,129||$9,623||$11,817|
|Y1H Consumables only per Promoter/Arabidopsis 800||$138||$217||$266|
|Y1H Training Arabidopsis 800||$4,581||$7,192||$8,832|
|Y1H Addt’l Screening/Arabidopsis 800||$1,449||$2,275||$2,794|
|Y1H Screening of 1-6 Promoters/Arabidopsis 2000||$6,705||$10,527||$12,927|
|Y1H Consumables only per Promoter/Arabidopsis 2000||$374||$588||$722|
|Y1H Training Arabidopsis 2000||$5,157||$8,096||$9,942|
|Y1H Addt’l Screening/Arabidopsis 2000||$1,629||$2,557||$3,140|
|Y1H Screening of 1-24 Promoters/ Maize 500||$6,561||$10,301||$12,650|
|Y1H Consumables only per Promoter/ Maize 500||$104||$164||$201|
|Y1H Training Maize 500||$4,797||$7,531||$9,248|
|Y1H Addt’l Screening/ Maize 500||$1,449||$2,275||$2,794|
|Labor Rate II||$36||$57||$69|
Burdo, B., J. Gray, M. P. Goetting-Minesky, B. Wittler, M. Hunt, T. Li, D. Velliquette, J. Thomas, I. Gentzel, M. dos Santos Brito, M. K. Mejia-Guerra, L. N. Connolly, D. Qaisi, W. Li, M. I. Casas, A. I. Doseff and E. Grotewold (2014). “The Maize TFome–development of a transcription factor open reading frame collection for functional genomics.” Plant J 80(2): 356-366.
Gaudinier, A., L. Zhang, J. S. Reece-Hoyes, M. Taylor-Teeples, L. Pu, Z. Liu, G. Breton, J. L. Pruneda-Paz, D. Kim, S. A. Kay, A. J. Walhout, D. Ware and S. M. Brady (2011). “Enhanced Y1H assays for Arabidopsis.” Nat Methods 8(12): 1053-1055.
Li, B., A. Gaudinier, M. Tang, M. Taylor-Teeples, N. T. Nham, C. Ghaffari, D. Benson, M. Steinmann, J. A. Gray, S. M. Brady and D. J. Kliebenstein (2014). “Promoter based integration in plant defense regulation.” Plant Physiol.
Pruneda-Paz, J. L., G. Breton, D. H. Nagel, S. E. Kang, K. Bonaldi, C. J. Doherty, S. Ravelo, M. Galli, J. R. Ecker and S. A. Kay (2014). “A genome-scale resource for the functional characterization of Arabidopsis transcription factors.” Cell Rep 8(2): 622-632.