UC Davis Genome Center Proteomics
Core Rates
Effective as of August 2008
Protein Identification from a gel band or solution
This includes
· A quality control run at 125 fmol
· Proteolytic digestion (Trypsin)
· database search
· results returned via Scaffold (http://www.proteomesoftware.com )
For more information on Bottom up Protein identification using LC/MS/MS see
Common questions are what service(s) do I need? Generally after you contact us and we discuss your sample and what you are looking for we recommend something from the list. If you want a general idea to estimate your cost before you contact us here are some general guidelines.
These are just general guidelines, but they should allow you to estimate your costs
- If you are looking for Post translational modifications we usually analyze those with the LTQ-FT
- If you expect less than 300-400 proteins we generally run LC/MS/MS
- If you want to identify more than 300-400 proteins we generally run LC/LC/MS/MS
Ion trap (LTQ) LC/MS/MS (30-90 minute run based on complexity of your sample) 152.00 LC/LC/MS/MS (mudpit 8 x 20 minute runs) 254.00 LC/LC/MS/MS (mudpit 8 x 90 minute runs) 358.00 FTMS (LTQ-FT) LC/MS/MS (30-90 minute run based on complexity of your sample) 179.00 LC/LC/MS/MS (mudpit 8 x 20 minute runs) 296.00 LC/LC/MS/MS (mudpit 8 x 90 minute runs) 399.00 *If you perform the digest, subtract 26.00 from the above prices LC/MS/MS ONLY ( no data analysis), mzXML file will be returned to customer, does not include sample prep. Recommended only for people that have experience interpreting LC/MS/MS data) LTQ or LTQ-FT (30-90 minute run) 70.00 Identification of a Post-tranlational Modification
- Protein Identification Rate + Data analysis time
Quantitative Proteomic Profiling (spectral counting or dMS)
- Protein Identification Rate per replicate + Data analysis Time
Targeted Proteomics (includes digest, usually 3-4 hours of data analysis are also required. Targeted proteomics projects are usually considered collaborative as designing the experiment is critical for success. Please contact us for details.
TSQ Vantage LC/MS/MS 152.00
Data Analysis/Labor Rate 68.00/hour
Other Sample Prep
Costs of Reagents + 68.00/hour
Things to keep in mind:
Quantitiave experiments usually require multiple runs plus considerable Data analysis time. Please contact us for specific details.
If you want to do a collaborative project with the Facility please contact us. Collaborative Projects are projects where we contribute significantly on large projects. This is recommended if you plan on doing things more complicated than simple proteins identification. For more information please see
and
Rates for non-profit institutions and Other Universities add 52.00%
Rates for commercial companies add 81.50%
